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Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area

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Author(s): Sara Jelodarian | Abdolrasoul Haghir Ebrahimabadi | Ahmad Khalighi | Hossain Batooli

Journal: Avicenna Journal of Phytomedicine
ISSN 2228-7930

Volume: 2;
Issue: 3;
Start page: 139;
Date: 2012;
Original page

Keywords: Apple | Malus domestica | Extract | Antioxidant activity | Total phenolic content

ABSTRACT
Objective: Antioxidants are considered as the main factors in the inhibition of unwanted oxidation reactions.Materials and Methods: In this research the antioxidant potential of the fresh fruits of 4 cultivars (A to D) of Malus domestica (M. domestica) cultivated in the Kashan, Qamsar area was evaluated. The antioxidant activity of the samples were evaluated using two complementary antioxidant assays: 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid tests and the results were compared with the synthetic standard antioxidant butylated hydroxytoluene (BHT). Results: Total phenolic contents of the samples are also estimated by Folin-Ciocalteu's phenol test. In both DPPH β-carotene/linoleic acid tests in the concentration of 2 mg/ml, only samples from cultivar A showed moderate antioxidant activity with 63.92±0.42 and 6.02±0.03 inhibition percentages, respectively and other samples were weakly active.Conclusion: The Folin-Ciocalteu's phenol test was also showed very little phenolic compounds for the fruits. In conclusion, weak antioxidant activity was estimated for the studied apple cultivars.
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