Author(s): Basel Saleh
Journal: Journal of Stress Physiology & Biochemistry
ISSN 1997-0838
Volume: 9;
Issue: 3;
Start page: 44;
Date: 2013;
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Keywords: AFLP marker | Cotton | Physiological parameters | Salt stress
ABSTRACT
This investigation aimed to evaluate five upland cotton (Gossypium hirsutum L.) (Aleppo118, Aleppo33/1, Aleppo90, Raqqa5, and Deir-Ezzor22) varieties based on physiological indices and genetic variation using AFLP marker. These varieties were evaluated under control and saline conditions (50, 100 & 200 mM NaCl) for 56 days. Various physiological indices were detected in this investigation. In this respect, Deir-Ezzor22 variety differed by showing high salt tolerance relative to the other tested varieties. Otherwise, PCR amplification with 7 AFLP PCs primer combinations revealed that Deir-Ezzor22 variety characterized by 15 unique positive markers compared to the other tested varieties. Based on physiological study and AFLP technique, it can be concluded that genetic variation detected by AFLP marker supported the physiological indices among the tested cotton varieties. These varieties present considerable interest forngenetic studies and plant improvement.
Journal: Journal of Stress Physiology & Biochemistry
ISSN 1997-0838
Volume: 9;
Issue: 3;
Start page: 44;
Date: 2013;
VIEW PDF
DOWNLOAD PDF Original pageKeywords: AFLP marker | Cotton | Physiological parameters | Salt stress
ABSTRACT
This investigation aimed to evaluate five upland cotton (Gossypium hirsutum L.) (Aleppo118, Aleppo33/1, Aleppo90, Raqqa5, and Deir-Ezzor22) varieties based on physiological indices and genetic variation using AFLP marker. These varieties were evaluated under control and saline conditions (50, 100 & 200 mM NaCl) for 56 days. Various physiological indices were detected in this investigation. In this respect, Deir-Ezzor22 variety differed by showing high salt tolerance relative to the other tested varieties. Otherwise, PCR amplification with 7 AFLP PCs primer combinations revealed that Deir-Ezzor22 variety characterized by 15 unique positive markers compared to the other tested varieties. Based on physiological study and AFLP technique, it can be concluded that genetic variation detected by AFLP marker supported the physiological indices among the tested cotton varieties. These varieties present considerable interest forngenetic studies and plant improvement.
