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Expression and localization of claudins-3 and -12 in transformed human brain endothelium

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Author(s): Schrade Anja | Sade Hadassah | Couraud Pierre-Olivier | Romero Ignacio A | Weksler Babette B | Niewoehner Jens

Journal: Fluids and Barriers of the CNS
ISSN 2045-8118

Volume: 9;
Issue: 1;
Start page: 6;
Date: 2012;
Original page

Keywords: Blood Brain Barrier (BBB) | hCMEC/D3 | Claudins | Tight junction | Statins

ABSTRACT
Abstract Background The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. Findings hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture. Conclusions These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro.
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