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GENETIC DIVERSITIES IN THE SIXTH - GENERATION OF SELECTION (S6) OF SOME INBRED LINES OF MAIZE BASED ON THE PHENOTYPIC CHARACTERS AND SSR

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Author(s): Heri Kustanto | Nur Basuki | Ariffin Noor Sugiharto | Astanto Kasno

Journal: Agrivita : Journal of Agricultural Science
ISSN 0126-0537

Volume: 34;
Issue: 2;
Start page: 127;
Date: 2012;
Original page

Keywords: genetic diversity | phenotype | molecular | inbred line | maize

ABSTRACT
The main objective of this research was to study the diversity of maize inbred lines based on phenotypic characters and SSR markers. The research, which was conducted from February to June 2011, was divided into field and laboratory research for molecular analysis. The molecular analysis was conducted at the Biotechnology Laboratory, Faculty of Agriculture, Brawijaya University Malang. The field trial was done in Kandat, Junrejo, Batu. Materials of the research were 35 genotypes of maize. Steps for molecular analysis included: (1) DNA isolation, (2) DNA-quality test, (3) PCR SSR, and (4) Visualization of the amplification result. The field trial was done by planting genotypes using a RCBD with 2 replications. Result of the dendogram analysis showed that 35 genotypes of maize were divided into 2 main clusters, A and B, that having 35% similarity level. Result for the analysis of variance showed significant difference among genotypes of maize on the entire of the observed phenotype characters. Heritability (H) ranged from 61.8% to 98.1%. Coefficient of variance ranged from 0.71 to 7.20%. Analysis on the genotypic correlation ranged from 0.06 to 1.0. Numbers of rows per ear showed significant correlation, in which Jaccard’s similarity coefficient (rs) = +0.43.
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