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Genetic Structure of Varroa Mite Populations in A. Mellifera syriaca

Author(s): Nizar Haddad, Hussein Migdadi, Jay Evans , Jeff Pettis

Journal: Advances in Environmental Biology
ISSN 1995-0756

Volume: 1;
Issue: 1;
Start page: 1;
Date: 2007;
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Jordan is considered as a transcendent area between the African and the Asian countries. To provide amore precise picture of the genetic structure of varroa populations in Jordan compare with world wide picture,a total of 60 female mites belong to 10 A.m ligustica colonies collected from north of Jordan and 20 mitesfrom A.m syriaca colonies collected from two locations; one from south where the bees are kept in traditionalwooden hives and the second from the north in which bees are kept in a clay mode hives. These twotraditional apiaries were found to be closest to the historical samples collected by Brother Adam in 1952.Using specific oligo nucleotide primers CO1F.F and CO1N.R and the amplified products were sequenced usingBig Dye 2.0 end- terminal cycle sequencing and analyzed on an Applied Biosystems 3130 DNA analyzer.Sixteen varroa mites collected from several sites in Jordan were successfully sequenced and compared to thosefrom Algeria, Bolivia, South Korea, Vietnam, China, Japan, Nepal and Sri Lanka. All Jordanian samples werematches with Korean haplotype of V.destructor. This is the first analysis of V.destructor types in Jordanparasitizing on A.m.syriaca. Further collection within A.m.syriaca can help confirm that the mites in thissubspecies are as with A.m ligustica predominantly the Korean haplotype.
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