Author(s): González-Galaviz José Reyes | Rodriguez-Anaya Libia Zulema | Molina-Garza Zinnia Judith | Ibarra-Gámez José Cuauhtémoc | Galaviz-Silva Lucio
Journal: Archives of Biological Sciences
ISSN 0354-4664
Volume: 65;
Issue: 3;
Start page: 945;
Date: 2013;
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Keywords: White spot syndrome virus | VNTR | SNP | ORF94 | ORF75 | ORF125
ABSTRACT
White spot syndrome is a viral disease affecting wild and farm crustaceans that serve as reservoirs. Previous reports have demonstrated high genomic variation in WSS viruses (WSSV) isolated from distinct geographical regions. In this study, we collected wild shrimps (Litopenaeus stylirostris), crabs (Callinectes arcuatus) and farmed shrimp (L. vannamei) in Sonora, Mexico, between 2008 and 2010. DNA was extracted, and the variable regions and transposase genes were subjected to PCR and sequencing. Compared to strains of WSSV from other sites, Mexican samples exhibited a distinct number of repeat units (RUs) in ORF94, ORF75 and ORF125, which ranged between 1-11, 3-15, and 8-11 RUs respectively, and a unique single nucleotide polymorphism (SNP) at position 48 of ORF94. A total of six Mexican genotypes were found in organism from shrimp farm and natural environment.
Journal: Archives of Biological Sciences
ISSN 0354-4664
Volume: 65;
Issue: 3;
Start page: 945;
Date: 2013;
VIEW PDF
DOWNLOAD PDF Original pageKeywords: White spot syndrome virus | VNTR | SNP | ORF94 | ORF75 | ORF125
ABSTRACT
White spot syndrome is a viral disease affecting wild and farm crustaceans that serve as reservoirs. Previous reports have demonstrated high genomic variation in WSS viruses (WSSV) isolated from distinct geographical regions. In this study, we collected wild shrimps (Litopenaeus stylirostris), crabs (Callinectes arcuatus) and farmed shrimp (L. vannamei) in Sonora, Mexico, between 2008 and 2010. DNA was extracted, and the variable regions and transposase genes were subjected to PCR and sequencing. Compared to strains of WSSV from other sites, Mexican samples exhibited a distinct number of repeat units (RUs) in ORF94, ORF75 and ORF125, which ranged between 1-11, 3-15, and 8-11 RUs respectively, and a unique single nucleotide polymorphism (SNP) at position 48 of ORF94. A total of six Mexican genotypes were found in organism from shrimp farm and natural environment.
