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Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA

Author(s): Cuong Cao | Sam W. Birtwell | Jonas Høgberg | Hywel Morgan | Anders Wolff | Dang Duong Bang

Journal: Diagnostics
ISSN 2075-4418

Volume: 2;
Issue: 4;
Start page: 72;
Date: 2012;
Original page

Keywords: gold nanoparticles | SU-8 | autofluorescence | solid-phase PCR amplification | DNA hybridization

SU-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (Bio-MEMS) or lab-on-a-chip (LOC) devices. However, SU-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse. Here, we show that deposition of a thin gold nanoparticles layer onto the SU-8 surface significantly reduces the autofluorescence of the coated SU-8 surface by as much as 81% compared to bare SU-8. Furthermore, DNA probes can easily be immobilized on the Au surface with high thermal stability. These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity. The approach is simple and easy to perform, making it suitable for various Bio-MEMs and LOC devices that use SU-8 as a structural material.
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