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INFLUENCE OF HEIRLOOM TOMATO POLYPHENOL EXTRACTS ON EXPRESSION OF INFLAMMATION GENES IN BOVINE

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Author(s): Kwaku Gyenai | Nona Mikiashvili | Hamid Ismail | Mulumebert Worku

Journal: American Journal of Animal and Veterinary Sciences
ISSN 1557-4555

Volume: 7;
Issue: 3;
Start page: 126;
Date: 2012;
Original page

Keywords: Bovine | Tomato Polyphenol | Transcription | Enzyme-Linked Immunosorbent Assay (ELISA) | Phosphate Buffered Saline (PBS)

ABSTRACT
The effect of tomato on diseases of economic importance continues to be of broad interest. To evaluate these relationships, the effect of crude extracts of Cherokee purple tomato on pro-inflammatory genes and cytokines were evaluated. Neutrophils from three Holstein Fresian cows were treated with Phosphate Buffered Saline (PBS) or E. coli O111-B4 Lipopolysaccharide (LPS). Treated cells were exposed to different concentrations of polyphenol extracts of fresh and heated Cherokee purple. Transcription of Cyclooxygenase-2 (Cox-2) and Tumor Necrosis Factor-alpha (TNF-α) was detected using real time PCR. Secretion of cytokines was evaluated using specific Enzyme-Linked Immunosorbent Assay (ELISA)’s for three pro-inflammatory genes. Transcription of Cox-2 and TNF-α in bovine neutrophils were found. No significant treatment effect of tomato polyphenols was found in the LPS treated neutrophils. TNF-α and Cox-2 expression in bovine neutrophils were modulated by polyphenol treatment. Variable concentration of polyphenol extracts had no effect on Cox-2 transcription. Gene expression analysis of TNF-α and Cox-2 mRNA showed significantly decreased transcription of Cox-2 in neutrophils exposed to polyphenol extracts (p

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