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Interpretation of Inotropic Effect Exhibited by Desmodium gangeticum Chloroform Root Extract Through GSMS and Atomic Mass Spectroscopy: Evaluation of its Anti Ischemia Reperfusion Property in Isolated Rat Heart

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Author(s): G.A. Kurian | R.S.S. Srivats | R. Gomathi | M.M. Shabi | J. Paddikkala

Journal: Asian Journal of Biochemistry
ISSN 1815-9923

Volume: 5;
Issue: 1;
Start page: 23;
Date: 2010;
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Keywords: kymogram | ischemic reperfusion | Desmodium gangeticum | gas spectroscopy/mass spectroscopy | atomic absorption

ABSTRACT
Desmodium gangeticum (DG) chloroform root extract was determined in frog and assessed in isolated rat heart. Ischemia reperfusion injury was experimentally induced in rat by using Langendroff apparatus and was perfused with Kreb Hanseleit buffer through aorta. Ischemia-reperfusion injury was induced by 30 min of global ischemia and 45 min of reperfusion in isolated rat hearts. Heart rate, coronary flow and Left Ventricular Pressure (LVP) were recorded and the activity of lactate dehydrogenase in coronary effluent and creatine kinase and lactate dehydrogenase contents in myocardial tissues were measured. Administration of DG root chloroform extract (20 mg kg-1 b.wt.) before global ischemia caused a significant improvement of cardiac function and a decrease in the release of lactate dehydrogenase in coronary effluent. Kymogram result in isolated frog heart showed that DG root extract possess positive inotropic effect. GSMS and atomic absorption analysis of DG root extract confirm the presence of bio-molecules that can stimulate the release of calcium in heart. From the above observations of present study, we can conclude that DG chloroform root extract can protect the myocardium against the damages induced by ischemia reperfusion in rats and the effect of the extract may be related to calcium releasing property.]]>
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