Author(s): Ali Sarani | Gholamreza Mohammadi | Ashraf Mayameei | Masoud Akbari
Journal: Human & Veterinary Medicine
ISSN 2066-7655
Volume: 5;
Issue: 1;
Start page: 29;
Date: 2013;
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Original page
Keywords: Equine herpesvirus | real-time PCR | virus quantitation
ABSTRACT
Objective: To detect the presence or absence of EHV-1 and EHV-4 in North-East equine population of Iran. Material and methods:Blood samples of 200 adult horses located in 80 different rural areas of North-East of Iran, were examined for Equine herpesvirus-1 and 4 presences.Absolute quantitation of EHV-4 target molecules was performed using standard curves and the detection limit of the assay was shownto be six copies per reaction. Results: Our study showed a high prevalence of EHV-4 (88%) in these regions. EHV-1 DNA was not detected inany sample. Conclusion: In addition to previous serological study, our report is the first to detect the EHVs in blood samples of Iran’s equinepopulation by using a high sensitive real-time PCR diagnostic assay and it provides new information for the virus distribution map.
Journal: Human & Veterinary Medicine
ISSN 2066-7655
Volume: 5;
Issue: 1;
Start page: 29;
Date: 2013;
VIEW PDF
DOWNLOAD PDF Original pageKeywords: Equine herpesvirus | real-time PCR | virus quantitation
ABSTRACT
Objective: To detect the presence or absence of EHV-1 and EHV-4 in North-East equine population of Iran. Material and methods:Blood samples of 200 adult horses located in 80 different rural areas of North-East of Iran, were examined for Equine herpesvirus-1 and 4 presences.Absolute quantitation of EHV-4 target molecules was performed using standard curves and the detection limit of the assay was shownto be six copies per reaction. Results: Our study showed a high prevalence of EHV-4 (88%) in these regions. EHV-1 DNA was not detected inany sample. Conclusion: In addition to previous serological study, our report is the first to detect the EHVs in blood samples of Iran’s equinepopulation by using a high sensitive real-time PCR diagnostic assay and it provides new information for the virus distribution map.
