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Isolation and Screening of Dye Decolorizing Bacterial Isolates from Contaminated Sites

Author(s): Anamika Pokharia | Sarabjeet Singh Ahluwalia

Journal: Textiles and Light Industrial Science and Technology
ISSN 2304-943X

Volume: 2;
Issue: 2;
Start page: 54;
Date: 2013;
Original page

Keywords: Biodegradation | Decolorization | Microtiter Plate | Reactive Dyes

Forty five bacterial strains were isolated from contaminated textile wastewater and soil, and then isolates were screened for their ability to decolorize textile dyes from aqueous solution. Initially twenty four bacterial isolates were screened based on their ability to decolorize a wide spectrum of dyes efficiently such as Black WNN, Blue FNR, Red FN2BL, Blue RC, TURQ Blue and Diresul RDT Black dye, by a rapid microtiter plate screening method. Among all isolates, NF-23 was found to decolorize maximum number of dyes followed by NF-22 and NF-21. NF-23 decolorized Black WNN (95%), Blue FNR (50%), Diresul RDT black (90%) and Red FN2BL (80%) after 72 h of incubation at pH-9 and temperature 35°C under anoxic condition. These results signify that bacterial isolates could effectively be used in development of alternative and eco-friendly method for decolorization and biodegradation of textile dyes from industrial effluent.
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