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Karyological and Electrophoretic Distinction between Sexes of Trichosanthes bracteata

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Author(s): Kanika Karmakar | Rabindra Kumar Sinha | Sangram Sinha

Journal: American Journal of Plant Sciences
ISSN 2158-2742

Volume: 04;
Issue: 03;
Start page: 494;
Date: 2013;
Original page

Keywords: Trichosanthes bracteata | Dioecious | Reducing and Non-Reducing SDS-PAGE

ABSTRACT
Chromosome studies and soluble protein profiles, fractionated by reducing and non-reducing SDS-PAGE, were carried out in dioecious Trichosanthes bracteata. Somatic chromosome no. 2n = 22 was recorded in both sexes. The karyotype of male and female plant shows high homogeneity and the absence of any heteromorphic pair of chromosomes negates the possibility of XY mechanism. Soluble protein profiles from the tuberous roots of the male and female plants, fractionated by reducing SDS-PAGE, did not show any qualitative distinction. Whereas the protein profile in non-reducing SDS-PAGE reveals a clear distinction when compared on a single gel. The difference is marked by the presence of a disulphide linked tertiary or folded protein at 19 k D region detected in male sex. However, at the level of primary structure the qualitative expression is similar indicating a common ancestry.
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