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LABORATORY DIAGNOSIS OF HUMAN BRUCELLOSIS: COMPARISON OF TWO TECHNIQUES OF SERUM AGGLUTINATION

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Author(s): K. Bellamine | M. Riyad | B.Takourt | B. Farouqi | H. Fellah

Journal: Technologies de Laboratoire
ISSN 1114-9981

Volume: 7;
Issue: 29;
Start page: 99;
Date: 2012;
Original page

Keywords: agglutination | brucellosis | antibodies | sensitivity | specificity

ABSTRACT
objective: Evaluate the technical characteristics of two agglutination techniques used for the detection of serum antibodies of Brucella.Material and methods: The search for Brucella antibodies was performed in 100 serum samples. They come from patients with brucellosis was suspected on clinical and epidemiological criteria. The average age was 31 years with a standard deviation of 1.5 and a sex ratio (M / W) is equal to 2. The two methods used are (manual): 1- BRUCELLACAPT® (Vircell,Spain)2- ROSE BENGAL ® (Bio-Rad, France).Results: The results showed a concordance between the two techniques of 92% (80% negative results and 12% positive), and discrepancy of 8%.Conclusion: Discordant results demonstrate the need to always integrate the approach diagnostic of a clinical and epidemiological data associated with biological data, and secondly, to take into account the threshold value of detection of serological markers of the technique used.
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