Academic Journals Database
Disseminating quality controlled scientific knowledge

Large-scale experimental studies show unexpected amino acid effects on protein expression and solubility in vivo in E. coli

Author(s): Price W Nicholson | Handelman Samuel K | Everett John K | Tong Saichiu N | Bracic Ana | Luff Jon D | Naumov Victor | Acton Thomas | Manor Philip | Xiao Rong | Rost Burkhard | Montelione Gaetano T | Hunt John F

Journal: Microbial Informatics and Experimentation
ISSN 2042-5783

Volume: 1;
Issue: 1;
Start page: 6;
Date: 2011;
Original page

Abstract The biochemical and physical factors controlling protein expression level and solubility in vivo remain incompletely characterized. To gain insight into the primary sequence features influencing these outcomes, we performed statistical analyses of results from the high-throughput protein-production pipeline of the Northeast Structural Genomics Consortium. Proteins expressed in E. coli and consistently purified were scored independently for expression and solubility levels. These parameters nonetheless show a very strong positive correlation. We used logistic regressions to determine whether they are systematically influenced by fractional amino acid composition or several bulk sequence parameters including hydrophobicity, sidechain entropy, electrostatic charge, and predicted backbone disorder. Decreasing hydrophobicity correlates with higher expression and solubility levels, but this correlation apparently derives solely from the beneficial effect of three charged amino acids, at least for bacterial proteins. In fact, the three most hydrophobic residues showed very different correlations with solubility level. Leu showed the strongest negative correlation among amino acids, while Ile showed a slightly positive correlation in most data segments. Several other amino acids also had unexpected effects. Notably, Arg correlated with decreased expression and, most surprisingly, solubility of bacterial proteins, an effect only partially attributable to rare codons. However, rare codons did significantly reduce expression despite use of a codon-enhanced strain. Additional analyses suggest that positively but not negatively charged amino acids may reduce translation efficiency in E. coli irrespective of codon usage. While some observed effects may reflect indirect evolutionary correlations, others may reflect basic physicochemical phenomena. We used these results to construct and validate predictors of expression and solubility levels and overall protein usability, and we propose new strategies to be explored for engineering improved protein expression and solubility.
Affiliate Program     

Tango Jona
Tangokurs Rapperswil-Jona