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Mapping of a SCAR marker tightly linked to the B mating type locus of Lentinula edodes (shiitake mushroom) by tetrad analysis

Author(s): A. Tanaka | K. Miyazaki | S. Shiraishi

Journal: Micología Aplicada Internacional
ISSN 1534-2581

Volume: 20;
Issue: 2;
Start page: 47;
Date: 2008;
Original page

Keywords: B mating-type locus | crossing over | Lentinula edodes | tetrad analysis

We isolated 132 basidiosporic strains of 33 tetrads from the outbred strains MCR14 and MCR15 of Lentinula edodes for linkage analyses. Mating tests detected crossing over between genes of the B mating-type locus during meiosis in four strains, MCR14B-121-3 (B3), MCR14B-121-4 (B4), MCR14B-130-1 (B3), and MCR14B-130-4 (B4), as monokaryons having different mating types from the parental type (B1 , B2). In MCR14B-121-3 and MCR14B-121-4, the recombination was also detected between a sequence characterized amplified region (SCAR) marker, sOPP19-560, tightly linked to the B mating-type locus and a random amplified polymorphic DNA (RAPD) marker, C02-0900t. Furthermore, sOPP19-560 mapped between the B mating-type locus and a RAPD marker, Y07-0820t

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