Author(s): A. Tanaka | K. Miyazaki | S. Shiraishi
Journal: Micología Aplicada Internacional
ISSN 1534-2581
Volume: 20;
Issue: 2;
Start page: 47;
Date: 2008;
Original page
Keywords: B mating-type locus | crossing over | Lentinula edodes | tetrad analysis
ABSTRACT
We isolated 132 basidiosporic strains of 33 tetrads from the outbred strains MCR14 and MCR15 of Lentinula edodes for linkage analyses. Mating tests detected crossing over between genes of the B mating-type locus during meiosis in four strains, MCR14B-121-3 (B3), MCR14B-121-4 (B4), MCR14B-130-1 (B3), and MCR14B-130-4 (B4), as monokaryons having different mating types from the parental type (B1 , B2). In MCR14B-121-3 and MCR14B-121-4, the recombination was also detected between a sequence characterized amplified region (SCAR) marker, sOPP19-560, tightly linked to the B mating-type locus and a random amplified polymorphic DNA (RAPD) marker, C02-0900t. Furthermore, sOPP19-560 mapped between the B mating-type locus and a RAPD marker, Y07-0820t
Journal: Micología Aplicada Internacional
ISSN 1534-2581
Volume: 20;
Issue: 2;
Start page: 47;
Date: 2008;
Original page
Keywords: B mating-type locus | crossing over | Lentinula edodes | tetrad analysis
ABSTRACT
We isolated 132 basidiosporic strains of 33 tetrads from the outbred strains MCR14 and MCR15 of Lentinula edodes for linkage analyses. Mating tests detected crossing over between genes of the B mating-type locus during meiosis in four strains, MCR14B-121-3 (B3), MCR14B-121-4 (B4), MCR14B-130-1 (B3), and MCR14B-130-4 (B4), as monokaryons having different mating types from the parental type (B1 , B2). In MCR14B-121-3 and MCR14B-121-4, the recombination was also detected between a sequence characterized amplified region (SCAR) marker, sOPP19-560, tightly linked to the B mating-type locus and a random amplified polymorphic DNA (RAPD) marker, C02-0900t. Furthermore, sOPP19-560 mapped between the B mating-type locus and a RAPD marker, Y07-0820t
