Author(s): Useni Reddy Mallu1 K. Hussain Reddy1, Varaprasad Bobbarala2*, Somasekhar Penumajji3,
Journal: Drug Invention Today
ISSN 0975-7619
Volume: 2;
Issue: 2;
Start page: 160;
Date: 2010;
Original page
Keywords: Amiodarone hydrochloride | anti-arrhythmic agent | High performance liquid chromatography (HPLC).
ABSTRACT
Amiodarone is an anti-arrhythmic agent used for various types of tachyarrhythmia both ventricular and supra ventricular arrhythmias (fast forms of irregular heart beat). Present study is aimed to develop simple, specific, sensitive, and rapid high performance liquid chromatography (HPLC) method for the determination of Amiodarone hydrochloride. Amiodarone was identified and quantitated on a C2 reversed phase column (4.6 × 250mm, 5.0µ), using a mobile phase composed of acetate buffer-Acetonitrile (15:85v/v) delivered at a flow rate of 1.0 mL/min, and with UV detection (lamda excitation= 240 nm). The method was proven to be linear over Amiodarone hydrochloride concentration range of 12.5 to 75µg/mL with a mean correlation coefficient of 0.9999. The present study is the development of HPLC method for the Pharmaceutical Formulation of Amiodarone Hydrochloride.
Journal: Drug Invention Today
ISSN 0975-7619
Volume: 2;
Issue: 2;
Start page: 160;
Date: 2010;
Original page
Keywords: Amiodarone hydrochloride | anti-arrhythmic agent | High performance liquid chromatography (HPLC).
ABSTRACT
Amiodarone is an anti-arrhythmic agent used for various types of tachyarrhythmia both ventricular and supra ventricular arrhythmias (fast forms of irregular heart beat). Present study is aimed to develop simple, specific, sensitive, and rapid high performance liquid chromatography (HPLC) method for the determination of Amiodarone hydrochloride. Amiodarone was identified and quantitated on a C2 reversed phase column (4.6 × 250mm, 5.0µ), using a mobile phase composed of acetate buffer-Acetonitrile (15:85v/v) delivered at a flow rate of 1.0 mL/min, and with UV detection (lamda excitation= 240 nm). The method was proven to be linear over Amiodarone hydrochloride concentration range of 12.5 to 75µg/mL with a mean correlation coefficient of 0.9999. The present study is the development of HPLC method for the Pharmaceutical Formulation of Amiodarone Hydrochloride.
