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THE MICROPROPAGATION OF SOME THORNLESS BLACKBERRY CULTIVARS

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Author(s): Clapa Doina | Alexandru Fira | Catita Plopa | A. Vescan

Journal: Scientific Papers of the Research Institute for Fruit Growing Pitesti, Romania
ISSN 1584-2231

Volume: XXVII;
Start page: 113;
Date: 2011;
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Keywords: micropropagation | cultivars | thornless blackberry

ABSTRACT
Our paper presents aspects regarding the in vitro propagation of three thornless blackberry cultivars: ’Loch Ness’, ’Smoothstem’ and ’Thornless evergreen’ for the production of planting material. The culture medium used had, as components, Murashige & Skoog 1962 (MS) salts, Myo inositol-100 mg/l, Vitamin B1- 1 mg/l, Vitamin B6 - 0.5 mg/l, Nicotinic Acid - 0.5 mg/l and, as growth regulator, 6-benzylaminopurine (BAP) at 0.7 mg/l for the in vitro culture initiation phase and 0.5 mg/l for the multiplication phase. The in vitro rooting phase was eliminated and the rooting of the shoots that resulted from the in vitro multiplication phase was done ex vitro in the same phase as acclimation, in float hydroculture. The ex vitro rooted and acclimated plants were transferred into potting mix, obtaining container-grown planting material.
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