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Modern Technologies of Bacterial Biofilm Study

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Author(s): I.V. Chebotar | A.G. Pogorelov | V.A. Yashin | E.L. Guryev | G.G. Lominadze

Journal: Sovremennye Tehnologii v Medicine
ISSN 2076-4243

Volume: 5;
Issue: 1;
Start page: 14;
Date: 2013;
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Keywords: Staphylococcus aureus | biofilm | Mass-spectrometry | Laser scanning confocal microscopy | Scanning electron microscopy

ABSTRACT
The aim of the investigation was to estimate the availability of new biomedical technologies to identify bacterial biofilms and evaluate them on a staphylococcal biofilm model.Materials and Methods. We studied staphylococcal biofilms by mass spectrometry, laser scanning (confocal) microscopy, scanning electron microscopy, enzymatic and oxidative destruction of extracellular biofilm matrix. Results. We demonstrated the capabilities of new biomedical technologies in identification of generic specificity of biofilm-forming staphylococcus, and in detection of the necessary characteristics of staphylococcal biofilm. Mass spectrometry enabled to identify the type of biofilm-forming staphylococcus (Staphylococcus aureus). Microscopic study using laser scanning confocal microscopic technique revealed 3-demensional organization typical of S. aureus biofilms. Scanning electron microscopy enabled to visualize the structures of extracellular S. aureus biofilm matrix. The extracellular matrix of the test biofilm was found to be formed of DNA-protein complexes.
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