Academic Journals Database
Disseminating quality controlled scientific knowledge

Molecular cloning and sequence analysis of cDNA encoding molt-inhibiting hormone from Portunus trituberculatus

ADD TO MY LIST
 
Author(s): ZHU Dong-Fa | SHEN Jian-Ming | YANG Ji-Feng | SU Qing

Journal: Acta Zoologica Sinica
ISSN 0001-7302

Volume: 54;
Issue: 6;
Start page: 1112;
Date: 2008;
Original page

Keywords: Portunus trituberculatus | Molt-inhibiting hormone (MIH) | cDNA cloning | Sequence analysis

ABSTRACT
Molt-inhibiting hormone (MIH), which belongs to the crustacean hyperglycemic hormone (CHH) neuropeptide family, inhibits the synthesis of ecdysteroids by Y-organs. The CHH family is divided into two major groups, designated I and II. Group Ⅰ is composed of all CHH peptides, and Group Ⅱ is composed of most MIHs, gonad-inhibiting hormone (GIH) and mandibular organ-inhibiting hormone (MOIH). The Group Ⅰ peptides consist of a signal sequence, a CHH precursor related peptide (CPRP) and a mature hormone. The Group Ⅱ peptides have only a signal sequence and a mature hormone. The full-length molt-inhibiting hormone cDNA of Portunus trituberculatus (Pot-MIH) was cloned by the reverse transcription-polymerase chain reaction (RT-PCR) and the rapid amplification of cDNA ends (RACE). The degenerate nucleotide primers was designed based on the amino acid sequence of other crustacean MIHs. The full-length sequence of Pot-MIH cDNA (GenBank accession number:EU284117) is 1572 bp in size and consists of a 210 bp 5′-untranslated region (5′-UTR), a 342 bp open reading frame and a 3′- untranslated region (3′-UTR). The deduced polypeptide consisted of a 78-amino acid mature MIH peptide and a 35-amino acid signal ptptide. The mature Pot-MIH peptide shared twelve conserved residues (Cys7, Gly12, Arg14, Cys24, Asp26, Cys27, Asn29, Arg32, Cys40, Cys44, Phe50, and Cys53) with other crustacean MIHs. The mature Pot-MIH peptide had the Gly12 residue and the precursor lacked a CPRP sequence, both of which are characteristic of all Group Ⅱ peptides. Alignment of the amino acid sequence deduced from the full-length Pot-MIH cDNA with MIH amino acid sequences of other crustaceans revealed that they had very high identity. The identities between the putative Pot-MIH amino acid sequence with that of MIH of P.pelagicus, Callinectes sapidus, Charybdis feriatus, Cancer pagurus, C.magister, Carcinus maenas and Gecarcinus lateralis were 97%, 95%, 90%, 82%, 81%, 77% and 60% respectively. Phylogenetic analysis indicates that the putative Pot-MIH cluster with other crustacean MIHs and GIHs [Acta Zoologica Sinica 54(6): 1112 –1118, 2008].
RPA Switzerland

RPA Switzerland

Robotic process automation

    

Tango Rapperswil
Tango Rapperswil