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Neuroblast migration and P2Y1 receptor mediated calcium signalling depend on 9-O-acetyl GD3 ganglioside

Author(s): Marcelo F Santiago | Eliana Scemes

Journal: ASN Neuro
ISSN 1759-0914

Volume: 4;
Issue: 6;
Start page: e00097;
Date: 2012;
Original page

Keywords: cerebellar granule cell | Jones antibody | GD3 synthase | P2Y1 receptor | calcium signalling and neuronal migration

Previous studies indicated that a ganglioside 9acGD3 (9-O-acetyl GD3) antibody [the J-Ab (Jones antibody)] reduces GCP (granule cell progenitor) migration in vitro and in vivo. We here investigated, using cerebellar explants of post-natal day (P) 6 mice, the mechanism by which 9acGD3 reduces GCP migration. We found that immunoblockade of the ganglioside with the J-Ab or the lack of GD3 synthase reduced GCP in vitro migration and the frequency of Ca2+ oscillations. Immunocytochemistry and pharmacological assays indicated that GCPs expressed P2Y1Rs (P2Y1 receptors) and that deletion or blockade of these receptors decreased the migration rate of GCPs and the frequency of Ca2+ oscillations. The reduction in P2Y1-mediated calcium signals seen in Jones-treated and GD3 synthase-null GCPs were paralleled by P2Y1R internalization. We conclude that 9acGD3 controls GCP migration by influencing P2Y1R cellular distribution and function.
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