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Ofloxacin analysis validation method in human blood plasma (in vitro) using solid-phase extraction HPLC

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Author(s): RESMI MUSTARICHIE | WIWIEK INDRIYAT | IYAN SOPYAN

Journal: Medical and Health Science Journal
ISSN 1804-1884

Volume: 8;
Issue: 4;
Start page: 80;
Date: 2011;
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Keywords: HPLC | ofloxacin | SPE | human plasma.

ABSTRACT
Until now, analysis of Ofloxacin in human blood plasma using solidphase extraction (SPE) by HPLC UV detector has not been reported. This study aims to determine the validity of analytical methods in Ofloxacin study in human blood plasma (in vitro) using an HPLC SPE UV detector. Plasma samples were extracted by SPE. Analytes were analyzed using a C18 column (octadecylsilane) 250x4.6 mm, particle size 10 0m, mobile phase 85,5:14,5 v v 0.025 M phosphate buffer (pH 2.2) and acetonitrile with a flow rate of 2 ml/min, detection performed at 294 nm with the internal standard ciprofloxacin. Validated analytical method was based on the parameters: selectivity, accuracy, precision, repeatability, linearity, LOD, LOQ, and the suitability of the system. Validation analysis showed selectivity test Rs>1.5, test repeatability with CV(%)
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