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Optimization of extracellular mannanase production from Penicillium oxalicum KUB-SN2-1 and application for hydrolysis property

Author(s): Sudathip Titapoka Chantorn | Katesarin Buengsrisawat | Apinya Pokaseam | Tasanee Sombat | Pichamon Dangpram | Kla Jantawon | Sunee Nitisinprasert

Journal: Songklanakarin Journal of Science and Technology
ISSN 0125-3395

Volume: 35;
Issue: 1;
Start page: 17;
Date: 2013;
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Keywords: Penicillium oxalicum | mannanase | copra meal | oligosaccharide production

Effects of media composition, and physical properties on the production of crude mannanase by Penicillium oxalicumKUB-SN2-1 were investigated. P.oxalicum KUB-SN2-1 was propagated in a shaking incubator at 30°C with rotation speed of200 rpm of 7 days. The specific activity obtained during growth on robusta coffee residues (RCR) of 16.21 U/mg protein wasmuch higher than other carbon sources tested. For nitrogen sources, yeast extract (0.11 U/mg protein) and ammonium nitrate(0.09 U/mg protein) showed maximum specific activity. Hence, guar gum was the best inducer for producing mannanase (14U/mg protein). For evaluating the optimal concentration, the result showed that 1% guar gum, 0.5% yeast extract, 0.25%ammonium nitrate, and 0.25% RCR were the suitable sources of inducer, organic nitrogen, inorganic nitrogen, and carbon,respectively. Modified medium with initial culture pH of 5.0 at 30°C was optimum for mannanase production (53.77 U/ml for3 day). Reducing sugars were analyzed by dinitrosalicylic acid methods. The highest reducing sugar of 7517.82 g/mlwas obtained from copra meal hydrolysate after 30 h.
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