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Phylogenetic Relationships among Amaranthus Species

Author(s): Sivagami Priya. P | Shyamala Kanakarajan

Journal: Advanced Biotech
ISSN 0973-0109

Volume: 13;
Issue: 03;
Start page: 11;
Date: 2013;
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Keywords: Amaranthus | Seed proteins | Analytical cut | SDS-PAGE | Cluster Analysis | UPGMA

The rst step towards improving the nutritional quality of Amaranthus, the total proteins were extracted from the seeds of three Amaranthus species [Amaranthus gangeticus (Mulai Keerai), Amaranthus dubius (Arai Keerai),Amaranthus polygonoides (Siru Keerai)] using two buffers --- Tris-HCl buffer (pH6.8); QB buffer (pH 7.8) with DTT and puried by analytically cut Ammonium Sulphate Precipitation method. The extracted and the precipitated seed proteins tested positive for their presence, estimated using Lowry's Method and run in SDSPAGE. The seed proteins and the precipitated seed proteins of three Amaranthus species tracted using two buffers, with different pH, showed distinct ymorphism in their electrophoretic banding patters with diverse molecular weight. This work reports the phylogenetic relationships between the seeds of three Amaranthusspecies based on Jaccard's similarity coefcient, using UPGMA. Two prominent clusters were noted two prominent clusters were noted (Cluster 1: Amaranthus gangeticus and Amaranthus lygonoides at one node. Cluster 2: Amaranthus dubius joined to the previously formed cluster). Their randomization was purely 100 %. Clustering of the genotypes signies close genetic proximity between / among species of different clusters, contrasting parents may be identied and used in the crossing programme for generating wider variability for the selection and crop improvement
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