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A Phytochemical Profile of Albizia lebbeck (L.) Benth. Cultivated in Egypt

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Author(s): S. El-Hawary | K. El-Fouly | N.M. Sokkar | Z. Talaat

Journal: Asian Journal of Biochemistry
ISSN 1815-9923

Volume: 6;
Issue: 2;
Start page: 122;
Date: 2011;
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Keywords: Albizia | DNA fingerprint | nutritives | tannins | analysis

ABSTRACT
Albizia lebbeck and the other species cultivated in Egypt was carried out for identification. Gravimetric determination of tannins in leaf and seed was found to be 4 and 5.3%, respectively and colorimetric determination of condensed tannins was 0.82 and 1.3%, respectively. Protein content, by Micro-Kjeldahl method, in different organs ranged from 7.49 and 25.48%. Amino acids, analyzed by HPLC-Pico-Tag method, were identified as 17 components in the seed, leaf and pericarp and 15 components in the stem; seed and leaf showed higher yields of essential (42.19 and 50.16%) and non essential (57.81 and 49.80%) amino acids, respectively. Results showed significant amount of essential amino acids above the recommended amount by WHO. Minerals investigation using atomic absorption spectrometry exhibited that potassium and sodium were major and amounted to 745.40 and 287.60 mg 100 g-1, respectively in seed and 660.90 and 253.50 mg 100 g-1, respectively in leaf, followed by calcium, magnesium and zinc. Vitamins analysis revealed that vitamin C, analyzed by 2, 6-dichloro-indophenol titrimetric method, was the major in the seed and leaf (56.80 and 37.04 mg 100 g-1, respectively) followed by -carotene, analyzed by HPLC/UV (210.50 and 209.80 g 100 g-1, respectively). GC/MS analysis of lipoidal matter showed that the methyl esters of fatty acids ranged from 46.40 to 52.30%. SaFA= 29.82-61.38%, MUFA= 15.46-30% and PUFA= 21.48-40.65%; linoleic acid was the major fatty acid in seed, leaf and pericarp (37.49, 32.72 and 20.28%, respectively). -Sitosterol was the major unsaponifiable component in seed and leaf ( 35.97 and 26.98%, respectively).]]>
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