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Phytochemical screening and antibacterial activity of crude ethanol root extract of Crotalaria pallida (Aiton) plant

Author(s): A Kwaji | R Atiko | Edeh Friday

Journal: Topclass Journal of Herbal Medicine
ISSN 2315-8840

Volume: 2;
Issue: 10;
Start page: 235;
Date: 2013;
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Keywords: Crotalaria pallida | phytochemical screening | antibacterial activity | MIC and MBC

The preliminary phytochemical screening of Crotalaria pallida (Aiton) ethanol root extract was carried out in accordance with standard procedures and the antibacterial activity was determined using agar well diffusion method. Phytochemical screening revealed the presence of steroids, glycosides, tannins, saponins, flavonoids, gums and reducing sugars. At a concentration of 400 mg/ml, the extract exhibited similar activity to chloramphenicol, a standard antibiotic. The minimum inhibitory concentration (MIC) for the microbes under test ranged from 12.50 mg/ml25.00 mg/ml of the crude extract, while the minimum bactericidal concentration (MBC) for the same set of organisms ranged from 50 mg/ml200 mg/ml of the extract. The study has shown that the ethanol root extract of C. pallida (Aiton) possess antibacterial activity which could be attributed to the presence of phytochemical substances
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