Author(s): M.A.R.Priyantha | N.Liyanagunawardana | A.A.Vipulasiri | P.S.Fernando | S.Rathakrishnen | G.A.Gunawardana
Journal: Wayamba Journal of Animal Science
ISSN 2012-578X
Volume: 4;
Issue: 1;
Start page: 285;
Date: 2012;
Original page
Keywords: HS | Cattle | vaccine | ISA | Montanide 206 VG | protection
ABSTRACT
Haemorragic septicemia is contagious disease, important disease in Sri Lanka specially in dry zone. Mass vaccination is carried out for three decades countinuosly and the disease has been controlledsuccessfully. Since the shelf life of mineral oil vaccine is limited the objective of the study was to compare the protection efficacy of “ISA Montanide 206 VG”,adjuvant instead of mineral oil in localcondition.The minimum lethal dose of Pasturella multocida was determined by the calves and protection efficacy of the two vaccinewere detected by the animal challenge experiment (n = 16).The antibody mediated immunity were monitored by indirect Haem agglutination test (IHA)both in disease free and endemic zone ofthe country. The lesion developments at the vaccinated site also were evaluated both at Bopaththalawa and Nikeweratiya. The minimum lethal dose of Pasturella multocida type B as determined as 104c.f.u./ ml subcutaneous route of administration and both adjuvant made vaccine given similar protection in potency test and IHA titters at two sites. In conclusion the ISA Montanide 206 VGwas accepted as better adjuvant for HS killed vaccine in mass scale vaccination together with protection efficacy and proven extended shelf life.
Journal: Wayamba Journal of Animal Science
ISSN 2012-578X
Volume: 4;
Issue: 1;
Start page: 285;
Date: 2012;
Original page
Keywords: HS | Cattle | vaccine | ISA | Montanide 206 VG | protection
ABSTRACT
Haemorragic septicemia is contagious disease, important disease in Sri Lanka specially in dry zone. Mass vaccination is carried out for three decades countinuosly and the disease has been controlledsuccessfully. Since the shelf life of mineral oil vaccine is limited the objective of the study was to compare the protection efficacy of “ISA Montanide 206 VG”,adjuvant instead of mineral oil in localcondition.The minimum lethal dose of Pasturella multocida was determined by the calves and protection efficacy of the two vaccinewere detected by the animal challenge experiment (n = 16).The antibody mediated immunity were monitored by indirect Haem agglutination test (IHA)both in disease free and endemic zone ofthe country. The lesion developments at the vaccinated site also were evaluated both at Bopaththalawa and Nikeweratiya. The minimum lethal dose of Pasturella multocida type B as determined as 104c.f.u./ ml subcutaneous route of administration and both adjuvant made vaccine given similar protection in potency test and IHA titters at two sites. In conclusion the ISA Montanide 206 VGwas accepted as better adjuvant for HS killed vaccine in mass scale vaccination together with protection efficacy and proven extended shelf life.
