Academic Journals Database
Disseminating quality controlled scientific knowledge

The quantitation of atorvastatin in human plasma by solid phase micro-extraction followed by LC-MS/MS and its application to pharmacokinetics study

Author(s): Pattarawit Rukthong | Polkit Sangvanich | Sarunyaporn Kitchaiya | Ekarat Jantratid | Korbtham Sathirakul

Journal: Songklanakarin Journal of Science and Technology
ISSN 0125-3395

Volume: 35;
Issue: 1;
Start page: 41;
Date: 2013;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: atorvastatin | LC-MS/MS | plasma | solid phase micro-extraction | pharmacokinetics

Determination of atorvastatin in human plasma using LC-MS/MS has been widely accepted as an effective methodowing to its practical applicability in routine drug analysis in pharmacokinetic and pharmacogenetic study. Nevertheless, themethod sensitivity can be compromised when limited sample volume is available. This problem is typically encountered inpharmacokinetics studies for which only a small volume of plasma sample can be obtained. Drug extraction from humanplasma was performed with the aid of reversed-phase C18 solid phase micro-extraction. The detection was accomplished byLC-MS/MS. The need of plasma volume of less than 250 ┬Ál for each sample made it possible to decrease the sample preparation time. The method was successfully validated and proved appropriate for the analysis of atorvastatin in human plasmaand can be applied to pharmacokinetics and pharmacogenetics study.
RPA Switzerland

Robotic Process Automation Switzerland


Tango Jona
Tangokurs Rapperswil-Jona