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Quantum dots nanoparticle-based lateral flow assay for rapid detection of Mycobacterium species using anti-FprA antibodies

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Author(s): Fabio Cimaglia | Alessandro Aliverti | Maurizio Chiesa | Palmiro Poltronieri | Enrico De Lorenzis | Angelo Santino | Leonardo A. Sechi

Journal: Nanotechnology Development
ISSN 2038-9671

Volume: 2;
Issue: 1;
Start page: e5;
Date: 2012;
Original page

Keywords: lateral flow | quantum dot | Mycobacterium tuberculosis | flavoprotein reductase A.

ABSTRACT
A lateral flow (LF) device combined with quantum dots (QDs) technology was developed for rapid detection of a specific mycobacterial flavoprotein reductase (fprA). In order to develop the LF assay based on a double-antibody sandwich format, two monoclonal antibodies recognizing different epitopes located in separated fprA domains were identified. The first monoclonal antibody was immobilized onto the detection zone of a porous nitrocellulose membrane, whereas another monoclonal antibody was conjugated to QDs nanoparticles as a detection system. Using these monoclonal antibodies we recorded a good fluorescence signal, the intensity of which was directly proportional to the concentration of fprA protein. The use of antibodies conjugated with fluorescent semiconductor QDs via biotin-streptavidin bridge, allowed the detection of fprA protein at concentrations as low as 12.5 pg/μL in less than 10 min. The reported technology could be useful in the diagnostic investigation of Mycobacterium tuberculosis and other human pathogens in clinical specimens.
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