Author(s): G.S. Nagananda | Nalini Satishchandra | S. Rajath
Journal: International Journal of Botany
ISSN 1811-9700
Volume: 7;
Issue: 4;
Start page: 310;
Date: 2011;
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Keywords: Flickingeria nodosa (Dalz.) Seidenf | CaCl2 | Burgeff?s N3F medium | PLBs | Sodium alginate
ABSTRACT
Flickingeria nodosa (Dalz.) Seidenf is an medicinally important epiphytic orchid. In this study, various concentrations of sodium alginate solutions and calcium chloride solutions were tested in order to optimize the shape, texture and time required for complexing of alginate synthetic seeds or beads for Flickingeria nodosa through encapsulation of Protocorm Like Bodies (PLBs). The developed PLBs on Burgeffs N3F basal medium were used as a source material for encapsulation. The sodium alginate was prepared with Burgeffs N3F basal medium which was used as a matrix for complexing. It was observed that 2% sodium alginate dipped in 100 mM CaCl2 solution and incubated for 30 min in orbital shaker was found to be the best matrix and complexing agent respectively to produce firm, transparent and uniform synthetic seeds. The synthetic seeds were stored at 4C for 3 months to study their viability. Further the encapsulated PLBs when cultured on 1X Burgeffs N3F medium, 2% sucrose fortified with 2 mg L-1 adenine sulphate and IAA 1 mg L-1 showed 95% germination response. The complete plantlets were successfully established with 85% survival frequency after a few days of indoor acclimatization and hardening. This technology can be adopted for ex-situ germplasm conservation of medicinally important vulnerable orchids.
Journal: International Journal of Botany
ISSN 1811-9700
Volume: 7;
Issue: 4;
Start page: 310;
Date: 2011;
VIEW PDF
DOWNLOAD PDF Original pageKeywords: Flickingeria nodosa (Dalz.) Seidenf | CaCl2 | Burgeff?s N3F medium | PLBs | Sodium alginate
ABSTRACT
Flickingeria nodosa (Dalz.) Seidenf is an medicinally important epiphytic orchid. In this study, various concentrations of sodium alginate solutions and calcium chloride solutions were tested in order to optimize the shape, texture and time required for complexing of alginate synthetic seeds or beads for Flickingeria nodosa through encapsulation of Protocorm Like Bodies (PLBs). The developed PLBs on Burgeffs N3F basal medium were used as a source material for encapsulation. The sodium alginate was prepared with Burgeffs N3F basal medium which was used as a matrix for complexing. It was observed that 2% sodium alginate dipped in 100 mM CaCl2 solution and incubated for 30 min in orbital shaker was found to be the best matrix and complexing agent respectively to produce firm, transparent and uniform synthetic seeds. The synthetic seeds were stored at 4C for 3 months to study their viability. Further the encapsulated PLBs when cultured on 1X Burgeffs N3F medium, 2% sucrose fortified with 2 mg L-1 adenine sulphate and IAA 1 mg L-1 showed 95% germination response. The complete plantlets were successfully established with 85% survival frequency after a few days of indoor acclimatization and hardening. This technology can be adopted for ex-situ germplasm conservation of medicinally important vulnerable orchids.
