Author(s): Janhavi Rao* | Kamini Sethy | Savita Yadav
Journal: Pharmacie Globale : International Journal of Comprehensive Pharmacy
ISSN 0976-8157
Volume: 02;
Issue: 04;
Start page: 1;
Date: 2011;
Original page
Keywords: Cefixime | Ofloxacin | High performance thin layer chromatography | validation.
ABSTRACT
This work is concerned with the simultaneous determination of cefixime and ofloxacin in a bulk drug and pharmaceutical formulations by high performance thin layer chromatographic (HPTLC) method. Chromatographic separation was achieved on aluminum foil plates precoated with silica gel 60GF-254, with n-butanol: ammonia: water: DMSO (8:3:1:2, v/v/v/v) as mobile phase. Detection was performed densitometrically at 297 nm. The RF of cefixime and ofloxacin were 0.55 and 0.65, respectively. The reliability of the method was assessed by evaluation of linearity (30-180 ng/spot for both cefixime and ofloxacin respectively. Accuracy (99.82 % for cefixime and 99.84 % for ofloxacin), and specificity, in accordance with ICH guidelines. The method is simple, accurate, and rapid and can therefore be used for routine analysis of both drugs in quality control laboratories.
Journal: Pharmacie Globale : International Journal of Comprehensive Pharmacy
ISSN 0976-8157
Volume: 02;
Issue: 04;
Start page: 1;
Date: 2011;
Original page
Keywords: Cefixime | Ofloxacin | High performance thin layer chromatography | validation.
ABSTRACT
This work is concerned with the simultaneous determination of cefixime and ofloxacin in a bulk drug and pharmaceutical formulations by high performance thin layer chromatographic (HPTLC) method. Chromatographic separation was achieved on aluminum foil plates precoated with silica gel 60GF-254, with n-butanol: ammonia: water: DMSO (8:3:1:2, v/v/v/v) as mobile phase. Detection was performed densitometrically at 297 nm. The RF of cefixime and ofloxacin were 0.55 and 0.65, respectively. The reliability of the method was assessed by evaluation of linearity (30-180 ng/spot for both cefixime and ofloxacin respectively. Accuracy (99.82 % for cefixime and 99.84 % for ofloxacin), and specificity, in accordance with ICH guidelines. The method is simple, accurate, and rapid and can therefore be used for routine analysis of both drugs in quality control laboratories.
