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Response of Explants of Calli Rice (Oryza sativa L.) Japonica cv. “Ilmi” to Gene Transformation Using Agrobacterium tumefaciens-Mediated

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Author(s): Fika Ayu Safitri | Mohammad Ubaidillah | Miswar &nbsp | Kyung-Min Kim

Journal: American Journal of Plant Sciences
ISSN 2158-2742

Volume: 04;
Issue: 04;
Start page: 838;
Date: 2013;
Original page

Keywords: AtBI-1 | Agrobacterium-Mediated | GFP | Transformation | Rice

ABSTRACT
The AtBI-1 gene encoding the Arabidopsis thaliana Bax inhibitor was introduced into Japonica cultivars of rice (“Ilmi”) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice calluses. Introduction of the AtBI-1 gene was also confirmed by PCR using AtBI-1 specific oligonucleotide primers in regenerated plants. Stable integration and expression of the AtbI-1 gene in plants were confirmed by GFP analysis.
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