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REZULTS OF MICROBIAL CONTAMINATION IN TISSUE CULTURE LABORATORY OF RIFG PITESTI

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Author(s): Valentina Isac | Camelia Cristea

Journal: Scientific Papers of the Research Institute for Fruit Growing Pitesti, Romania
ISSN 1584-2231

Volume: XXVII;
Start page: 129;
Date: 2011;
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Keywords: tissue culture | microbial contamination | bacterial and fungal isolates detection

ABSTRACT
Tissue culture contamination is a constant problem which frequently originates with the introduction into culture of explants contaminated with endophytic micro-organisms or surface sterilization-resistant micro-organisms. Heterotrophic plant tissue culture media are capable of supporting the growth of many common environmental micro-organisms. Microbial contaminations often affect very severely tissue culture studies and results. Microbes which contaminate the cultures and the laboratory environment and equipments are species of bacteria, fungus and viruses. This study aimed the investigation and identification of microbial contamination in tissue culture laboratory of RIFG Pitesti. Eight microbial contaminants (consisting of four fungi and four bacteria) were found associated with the laboratory environments and the tissue culture plants. The bacterial contaminations include Staphylococcus species, Streptococcus spp., Lactobacillus and Enterobacter spp. Fungal contaminants like Penicillium spp., Penicillium citrin and Aspergillus spp., were found associated with indoor air. Yeast Geotrichum was also found associated with tissue culture plants.
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