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Rhizobacteria Selection to Enhance Spore Germination and Hyphal Length of Arbuscular Mycorrhizal Fungi in Vitro

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Author(s): Cecep Hidayat | Dedeh H. Arief, Ane Nurbaity and Jajang Sauman

Journal: Asian Journal of Agriculture and Rural Development
ISSN 2304-1455

Volume: 3;
Issue: 4;
Start page: 199;
Date: 2013;
Original page

Keywords: AMF | Glomus sp | Hyphal length | MHB | Spore germination

ABSTRACT
In natural condition, Arbuscular Mycorrhizal Fungi (AMF) are surrounded by bacteria that help fungi symbiosis. The research aimed to get rhizobacteria that can act as Mycorrhiza Helper Bacteria (MHB) had been held at Soil Biology and Biotechnology Laboratory Faculty of Agriculture Unpad from February to March 2012. The experimental design used was completely randomized design with 11 treatments (bo= without rhizobacteria, b1= Pseudomonas diminuta, b2 = Bacillus alvei, b3 = B. mycoides, b4 = P. malei, b5= P. diminuta + B. alvei, b6 = P. diminuta + B. mycoides, b7 = P. diminuta + P. malei, b8 = B. alvei + B. mycoides, b9 = B. alvei + P. malei, b10= B. mycoides + P. malei) with 3 replications. Parameters evaluated were spore germination percentage and hyphal length of Glomus sp at 7, 14, 21, and 28 day after planting. The result showed that P. diminuta enhanced spore germination percentage and hyphal length of Glomus spas much as 224 % and 330%respectively than control. So, P. diminuta can be used as MHB.

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