Author(s): Bhukya Bhima, Sudhakara Reddy Marrivada, Tangutur Anjana Devi , Yerradoddi Ramana Reddy, and Linga Venkateswar Rao*
Journal: The IIOAB Journal
ISSN 0976-3104
Volume: 1;
Issue: 4;
Start page: 32;
Date: 2010;
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Keywords: Probiotics yeast | characterization | stresstolerance | animals
ABSTRACT
Based on the colony morphology and microscopic characteristics, 26 yeasts were isolated from different sources including brewery effluents. Initially they were screened for their thermotolerance at 40 0C and only 5 strains were selected. They were later grown in yeast extract peptone dextrose medium to screen their stress tolerance at five different temperatures; at different concentrations of a mixture of acetic, propionic and butyric acids; at different pH; at different concentrations of glucose and bile salts. Based on the growth at different stress conditions, yeast OBV9 was selected and characterized as Saccharomyces cerevisiae by sequencing its 5.8S rRNA gene and internal transcribed spacer (ITS) 1 and 2. The sequence obtained was most similar (99%) to S. cerevisiae, when it was blast searched in NCBI database and showed a separate branch in phylogenetic analysis.
Journal: The IIOAB Journal
ISSN 0976-3104
Volume: 1;
Issue: 4;
Start page: 32;
Date: 2010;
VIEW PDF
DOWNLOAD PDF Original pageKeywords: Probiotics yeast | characterization | stresstolerance | animals
ABSTRACT
Based on the colony morphology and microscopic characteristics, 26 yeasts were isolated from different sources including brewery effluents. Initially they were screened for their thermotolerance at 40 0C and only 5 strains were selected. They were later grown in yeast extract peptone dextrose medium to screen their stress tolerance at five different temperatures; at different concentrations of a mixture of acetic, propionic and butyric acids; at different pH; at different concentrations of glucose and bile salts. Based on the growth at different stress conditions, yeast OBV9 was selected and characterized as Saccharomyces cerevisiae by sequencing its 5.8S rRNA gene and internal transcribed spacer (ITS) 1 and 2. The sequence obtained was most similar (99%) to S. cerevisiae, when it was blast searched in NCBI database and showed a separate branch in phylogenetic analysis.
