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Silencing of the integrin-linked kinase gene suppresses the proliferation, migration and invasion of pancreatic cancer cells (Panc-1)

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Author(s): Xiang-Yu Zhu | Ning Liu | Wei Liu | Shao-Wei Song | Ke-Jian Guo

Journal: Genetics and Molecular Biology
ISSN 1415-4757

Volume: 35;
Issue: 2;
Start page: 538;
Date: 2012;
Original page

Keywords: E-cadherin | epithelial-mesenchymal transition (EMT) | integrin-linked kinase (ILK) | Panc-1 cell line | RNA interference (RNAi)

ABSTRACT
Integrin-linked kinase (ILK) is an ankyrin repeat-containing serine-threonine protein kinase that is involved in the regulation of integrin-mediated processes such as cancer cell proliferation, migration and invasion. In this study, we examined the effect of a lentivirus-mediated knockdown of ILK on the proliferation, migration and invasion of pancreatic cancer (Panc-1) cells. Immunohistochemical staining showed that ILK expression was enhanced in pancreatic cancer tissue. The silencing of ILK in human Panc-1 cells led to cell cycle arrest in the G0/G1 phase and delayed cell proliferation, in addition to down-regulating cell migration and invasion. The latter effects were mediated by up-regulating the expression of E-cadherin, a key protein in cell adhesion. These findings indicate that ILK may be a new diagnostic marker for pancreatic cancer and that silencing ILK could be a potentially useful therapeutic approach for treating pancreatic cancer.

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