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In silico Analysis and Homology Modeling of Putative Hypothetical Protein Q4QH83 of Leishmania major

Author(s): Kabila Venkatasamy

Journal: Advanced Biotech
ISSN 0973-0109

Volume: 13;
Issue: 03;
Start page: 01;
Date: 2013;
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Keywords: In-silico analysis | homology modeling | functional annotation | putative hypothetical protein | Leishmania majo

Genome sequencing studies has led to an explosion of large number of gene products and about half the proteins in most genomes are candidates for hypothetical proteins with unknown function. Leishmania major is an intracellular parasite causing cutaneous leishmaniasis in humans which targets dendritic cells and macrophages. The selected hypothetical protein of L.major (Q4QH83) was analysed and modeled using various bioinformatics tools and databases. The modeled protein revealed it is a stable, cellular protein with the presence of maximum number of coils as secondary structural elements. The presence of the DUF1861 domain suggests further studies are required to know more about its functional importance in organism specic niches or common biological role . The result of the present study suggests that the query protein has glycosyl hydrolase vebladed propellor domain and placed under Arbinanase/Levansucrase/Invertase superfamily. As the query resembles the template sequence of more than 70%, it might have a role in carbohydrate metabolism like the template and this needs further investigation. The protein model generated was found to be of fairly good quality with nearly 90% of the residues in favourable region. Since the protein under study was found to be cytoplasmic and stabe with cellular location , a comprehensive sequence and domain analysis is required which will throw more light of its role in carbohydrate metabolism and drug-target identication.

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