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A Simple and Sensitive RP-HPLC Method for Estimation of Lycopene in Pharmaceutical Solid Dosage Forms

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Author(s): Shantaram Nangude | Manisha Vite

Journal: Journal of Pharmaceutical Science and Bioscientific Research
ISSN 2277-3681

Volume: 3;
Issue: 1;
Start page: 16;
Date: 2013;
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Keywords: RP-HPLC | Lycopene | Validation

ABSTRACT
An RP-HPLC method was developed and validated for the estimation of Lycopene in bulk drug and solid dosage forms. The chromatographic system was equipped with Inertsil ODS-3V, C-18, 150 X 4.6mm internal diameter with 5 micron particle size column and PDA detector set at 472 nm, in conjunction with a mobile phase of Methanol, Tetra Hydro Furan and Water in the ratio of 66:30:4 % v/v at a flow rate of 1.5 ml/min. The retention time of Lycopene was found to be 6.805 minute. The separation was performed at ambient temperature. Linearity was observed in the concentration range of 10-80µg/ml with correlation co-efficient 0.9997 and slope 78219.2.Percentage recovery obtained 99.92-100.14 %. The percentage Assay was found to be 100.51 to 102.64 %.The proposed method is precise, accurate, selective and rapid for the determination of lycopene in bulk drug and solid dosage forms. The proposed method is optimized and validated as per the International Conference on Harmonization (ICH) guidelines
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