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Simultaneous Determination of Olmesartan Medoxomil and Hydrochlorothiazide by Area Under Curve and Dual Wavelength Spectrophotometric Methods

Author(s): K. Ilango | ,Shiji Kumar.P.S | P. P. K Joffa

Journal: Journal of Pharmaceutical Sciences and Research
ISSN 0975-1459

Volume: 4;
Issue: 10;
Start page: 1946;
Date: 2012;
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Keywords: Olmesartan medoxomil | Hydrochlorothiazide | AUC method | Dual wavelength method | Method validation

Two simple, accurate and reproducible spectrophotometric methods have been developed and validated for simultaneous estimation of olmesartan medoxomil and hydrochlorothiazide in combined dosage form. Olmesartan medoxomil shows maximum absorbance at 255 nm and hydrochlorothiazide shows maximum absorbance at 270 nm. For area under curve method, the wavelengths ranges between 250 – 260 nm and 265 – 275 nm were selected with reference to absorbance curves plotted between 200 – 400 nm. In dual wavelength method, two wavelengths were selected for each drug in a way so that the difference in absorbance is zero for another drug. Olmesartan medoxomil shows equal absorbance at 242 and 263 nm, where the difference in absorbance was measured for determination of hydrochlorothiazide. Similarly, difference in absorbance at 253 and 284 nm were measured for determination of olmesartan medoxomil. Linearity for detector response was observed in the concentration range of 5 – 40 µg/mL for olmesartan medoxomil and 3 – 24 µg/mL for hydrochlorothiazide for method I, 4 – 32 µg/mL for olmesartan medoxomil and 2.5 – 20 µg/mL for hydrochlorothiazide for method II respectively. Accuracy and precision studies were carried out and results were satisfactory. The proposed methods were validated as per ICH guidelines

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