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Statistical analysis of cellulase production in Bacillus amyloliquefaciens UNPDV-22

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Author(s): Vasudeo Zambare | Lew Christopher

Journal: Extreme Life, Biospeology & Astrobiology
ISSN 2066-7671

Volume: 3;
Issue: 1;
Start page: 38;
Date: 2011;
Original page

Keywords: Cellulase production | Bacillus amyloliquefaciens | optimization | response surface methodology | central composite design

ABSTRACT
The production of cellulase in Bacillus amyloliquefaciens UNPDV-22 was optimized usingresponse surface methodology (RSM). Central composite design (CCD) was used to study the interactiveeffect of fermentation medium components (wheat bran, soybean meal, and malt dextrin) on cellulaseactivity. Results suggested that wheat bran, soybean meal, and malt dextrin all have significant impacton cellulase production. The use of RSM resulted in a 70% increase in the cellulase activity over thecontrol of non-optimized basal medium. Optimum cellulase production of 11.23 U/mL was obtained in afermentation medium containing wheat bran (1.03%, w/v), soybean meal (2.43%, w/v), and maltdextrin (2.95%, w/v).

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