Author(s): Imani Fooladi A A
Journal: Medical Laboratory Journal
ISSN 1735-9007
Volume: 5;
Issue: 1;
Start page: 31;
Date: 2011;
Original page
Keywords: Anaerobic infection | Necrotic tooth | Pulp | preapical diseases
ABSTRACT
Background and objectives: Anaerobic microorganisms, particularlybacteroides, play an important role in causing Pulp and preapical diseases thatlead to chronic abscess. We aimed at studying anaerobic infections in necroticteeth having chronic clinical symptoms.Material and Methods: We examined 28 necrotic teeth of 28 patients for thepurpose of anaerobic culture. Fifteen cases with acute clinical symptoms and13 ones with chronic clinical symptoms. A Total of 38 Root canals from 28teeth were subjected for examination and sampling. The samples were culturedon Tayo glycolate medium, sent to laboratory and used the methods of Jar andGas pak for identification of bacteria.Results: The results indicate that 76% of isolated bacteria from necrotic canalsare obligate anaerobes and 24% are facultative anaerobes. The most commonisolated microorganisms are peptostreptococci, fusobacterium and bacteroides.When preapical problems are demonstrated by radiography, the same generaare found.Conclusion: These results are in agreement with the results of otherresearchers and it has been determined that bacteroids play an important rolein destroying the necrotic teeth's bone. Anaerobic bacteria causing dentalinfections are less identified and diagnosed; therefore, dentist and clinicalLaboratories should take this into account.Key words: Anaerobic infection, Necrotic tooth, Pulp, preapical diseases
Journal: Medical Laboratory Journal
ISSN 1735-9007
Volume: 5;
Issue: 1;
Start page: 31;
Date: 2011;
Original page
Keywords: Anaerobic infection | Necrotic tooth | Pulp | preapical diseases
ABSTRACT
Background and objectives: Anaerobic microorganisms, particularlybacteroides, play an important role in causing Pulp and preapical diseases thatlead to chronic abscess. We aimed at studying anaerobic infections in necroticteeth having chronic clinical symptoms.Material and Methods: We examined 28 necrotic teeth of 28 patients for thepurpose of anaerobic culture. Fifteen cases with acute clinical symptoms and13 ones with chronic clinical symptoms. A Total of 38 Root canals from 28teeth were subjected for examination and sampling. The samples were culturedon Tayo glycolate medium, sent to laboratory and used the methods of Jar andGas pak for identification of bacteria.Results: The results indicate that 76% of isolated bacteria from necrotic canalsare obligate anaerobes and 24% are facultative anaerobes. The most commonisolated microorganisms are peptostreptococci, fusobacterium and bacteroides.When preapical problems are demonstrated by radiography, the same generaare found.Conclusion: These results are in agreement with the results of otherresearchers and it has been determined that bacteroids play an important rolein destroying the necrotic teeth's bone. Anaerobic bacteria causing dentalinfections are less identified and diagnosed; therefore, dentist and clinicalLaboratories should take this into account.Key words: Anaerobic infection, Necrotic tooth, Pulp, preapical diseases