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Synergistic Effects of Glutathione and Vitamin E on ROS Mediated Ethanol Toxicity in Isolated Rat Hepatocytes

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Author(s): Ankush Gupta | Sangram Singh | Farrukh Jamal | Som Nath | Sudhir Mehrotra | Bechan Sharma

Journal: Asian Journal of Biochemistry
ISSN 1815-9923

Volume: 6;
Issue: 4;
Start page: 347;
Date: 2011;
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Keywords: oxidative stress | Ethanol | rotenone | hepatotoxicity | antioxidants

ABSTRACT
50) was found to be 20 mM of ethanol treated with rotenone (10 M) for 2 h. Treatment of ethanol with rotenone (20 mM10 M) caused increase in lipid peroxidation, increase of lactate dehydrogenase and decrease in reduced glutathione content. Reduced glutathione and a-tocopherol, alone and in combination, were added to hepatocyte suspension in an attempt to protect cells against alcohol induced cell damage. Pretreatment with reduced glutathione and vitamin E independently, increased the cell viability to 78.97 and 70.42%, respectively, but a combination of reduced glutathione and vitamin E increased cell viability to 92.12%. Vitamin E reduced lipid peroxidation by 41.32% but in combination with reduced glutathione decreased lipid peroxidation by 61.15%. The results suggest an interaction between GSH and Vitamin E in protecting ethanol induced hepatocytes damage.]]>
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