Academic Journals Database
Disseminating quality controlled scientific knowledge

Synthèses enzymatiques de néoglucoconjugués catalysées par l'alpha-glucosidase purifiée de la blatte Periplaneta americana (Linnaeus)

ADD TO MY LIST
 
Author(s): Kouamé LP. | Dué EA. | Niamké SL. | Kouamé FA. | Kamenan A.

Journal: Biotechnologie, Agronomie, Société et Environnement
ISSN 1370-6233

Volume: 9;
Issue: 1;
Start page: 35;
Date: 2005;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: α-glucosidase | transglucosylation | cockroach | Periplaneta americana

ABSTRACT
Enzymatic synthesis of neoglucoconjugates by purified α-glucosidase from cockroach Periplaneta americana (Linnaeus). Cockroach Periplaneta americana (Linnaeus) contains in his digestive tract an acid (pH 5,0) and mesophile (50°C) α-glucosidase. This enzyme, purified to homogeneity, hydrolyses highly maltose, sucrose and p-nitrophenyl-α-Dglucopyranoside. The ability of α-glucosidase from cockroach purified to homogeneity to catalyse transglucosylation reactions was tested using maltose and saccharose as glucosyl donors and 2-phenylethanol and phenol as acceptors. The experimental conditions were optimized in relation to the time course of the reaction, pH and concentrations of glucosyl donors and acceptors. The yields in transglucosylation reactions at 37 °C were very high and could attain 67% and 48% with 2-phenylethanol and phenol respectively as glucosyl acceptors. This α-glucosidase hydrolyzed the products formed. It seems that the products formed were the phenylethyl-α-D-glucoside and phenyl-α-D-glucoside. These results suggest that α- glucosidase from cockroach is an exoglucosidase which catalyse the splitting of the α-glucosyl residue from the non reducing terminal of the substrate to liberate α-glucose. This comportment indicates that this enzyme operated by a mechanism involving the retention of the anomeric configuration. On the basis of this work, α-glucosidase from P. americana appears to be a valuable tool for the preparation of α-neoglucoconjugates.

Tango Jona
Tangokurs Rapperswil-Jona

    
RPA Switzerland

RPA Switzerland

Robotic process automation