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Total phenolic content, antioxidant and antimicrobial activities of Blepharis edulis extracts

Author(s): Mohaddese Mahboubi | Ghasem Haghi | Nastaran Kazempour | Ali Reza Hatemi

Journal: Songklanakarin Journal of Science and Technology
ISSN 0125-3395

Volume: 35;
Issue: 1;
Start page: 11;
Date: 2013;
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Keywords: Blepharis persica | Blepharis edulis | phenolic total content | antimicrobial activity | antioxidant activity

Blepharis edulis is traditionally used as an antiseptic, purgative, aphrodisiac and anti-inflammatory agent. The extractsof plant aerial parts were screened for total phenolic content (TPC) gallic acid equivalents (GAE), total flavonoid compound(TFC) quercetin equivalents (QE), antioxidant capacity and its antimicrobial activity by micro broth dilution assay. The 50%-inhibition values of BHT and 70% (v/v) aqueous ethanol, 70% (v/v) aqueous methanol, methanol, and water extracts of B.edulis according to the DPPH method were found to be 19.6, 71.2, 73.7, 81.4, and 218.4 g/ml, respectively. TPC ranged from38.9 to 102.7 mg GAE/g dry extracts. The antimicrobial activity showed that yeast and fungi were sensitive and resistantmicroorganisms to the extracts. The 70%-methanol extract showed more drastic antimicrobial activity than the others. Theantimicrobial activity of ethanolic extract is the same as of the methanolic extract; water extract had the weakest antimicrobialactivity.

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