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In vitro capacity and in vivo antioxidant potency of sedimental extract of Tinospora cordifolia in streptozotocin induced type 2 diabetes

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Author(s): Ramachandran Kannadhasan | Subramaniam Venkataraman

Journal: Avicenna Journal of Phytomedicine
ISSN 2228-7930

Volume: 3;
Issue: 1;
Start page: 7;
Date: 2012;
Original page

Keywords: Antioxidants | Sedimental Extract of Tinospora Cordifolia (SETc) | Streptozotocin (STZ) | Type 2 Diabetes

ABSTRACT
Objective: The role of herbs against the free radicals have been put forth recently in combating many diseases. The aim of this study was to elucidate the in vitro capacity and in vivo antioxidant properties of sedimental extract of Tinospora cordifolia (SETc). Materials and Methods: SETc was subjected to in vitro chemical analysis such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide, hydrogen peroxide, and superoxide anion radicals scavenging respectively and finally drugs reductive ability in order to elucidate the antioxidant capacity of the test drug before introducing it into the biological membrane. The resulting capacity was evaluated in vivo by analyzing enzymic (SOD, CAT) and non-enzymic (vitamin C & E) antioxidant levels in the homogenized samples of major organs isolated from streptozotocin induced type 2 diabetic rats after 30th day of SETc (1000 mg/kg/p.o.) treatment. Finally, the histopathological evaluation was done using cut portion of the respective organs prone to free radical mediated cell destruction with STZ in order to study their micro anatomical changes.Results: Chemical analysis with SETc in vitro for its IC50 proves a key evident for its total antioxidant capacity of around 2046 times, in 1000 mg/kg of fixed dose per oral for in vivo analysis. In contrast to the above, the lipid peroxide levels and in vivo enzymic and non-enzymic antioxidant levels were found to possess most significant difference (p

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